CHOLESTEROL BIOSENSOR USING SINGLE CHOLESTEROL BIOSENSOR USING SINGLE LAYER GRAPHENE-NiO HYBRID COMPOSITELAYER GRAPHENE-NiO HYBRID COMPOSITE

Arunkumar Rengaraja , Yuvaraj Haldoraib, Seungbae Ahnc, Ki-Joon Jeonc, Young-Kyu Hanb, Yun Suk Huna*

aDepartment of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Incheon, 402-751, Republic of Korea

bDepartment of Energy and materials Engineering, Dongguk university-Seoul, Seoul, Republic of KoreaaDepartment of Environmental Engineering, Inha University, Incheon, 402-751, Republic of Korea

Corresponding Author. Tel: +82 32 860 9177, E-mail: yunsuk.huh@inha.ac.kr

Abstract

Fabrication of NiO/Graphene Hybrid Structure

Scheme 1. Schematic diagram of NiO electrodepostion on CVD grown Graphene

Electrochemical characterization

Figure 2.(a) I-V curve for Graphene & Graphene-NiO Hybrid Structure in KOH , b) I-V curve for Graphene-NiO at various

scan rate in KOH .

Figure 3. (a) ) I-V curve for Graphene/NiO at different concentration of cholesterol in KOH , b) Chronoamperometric

analysis of cholesterol concentration

Figure 1. (a) Raman spectrum of NiO/graphene nanocomposite, b) 2D band of graphene fitting with a single Lorentzian peak centered at 2679 cm–1, c) XRD pattern of nanocomposite and d) XPS

Acknowledgments

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean

Ministry of Science, ICT & Future Planning (2010-C1AAA001-0029018) and the Basic Science Research

Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT

& Future Planning (No. 2014R1A5A1009799).

Cholestrol sensing property of Graphene-NiO hybrid structure

Figure 4.(a) Selectivity of the composite towards cholesterol b) Stability of the composite towards cholesterol

sensing.

Selectivity and stability

Conclusion

We successfully fabricated a NiO/graphene nanocomposite by

electrodeposition of NiO onto the surface of CVD-grown graphene

Composite electrode detected a range of cholesterol concentrations from 2

mm to 40 mm

Low detection limit and rapid response time of 0.13 mm and 5 s,

Interference materials (AA, UA, uracil, and NaCl) exerted no significant effects

High stability and shelf-life

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