homework: complete step 4 (summary) on intro to enzymes worksheet silent do now:
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Bio 9A/9D: Tuesday, 11.2.10 Title : Introduction to Enzymes!. Homework: Complete Step 4 (Summary) on Intro to Enzymes worksheet Silent Do Now: Silently pass your typed McMush Lab Report forward - PowerPoint PPT PresentationTRANSCRIPT
Homework: Complete Step 4 (Summary) on Intro to
Enzymes worksheet Silent Do Now:
Silently pass your typed McMush Lab Report forward
(on the Intro to Enzymes handout) What comes to mind when you hear the word enzyme? What do you think an enzyme is?
Today’s Objectives: Describe what an enzyme does and how it
works Explain why an enzyme’s shape is so
important
Bio 9A/9D: Tuesday, 11.2.10Title: Introduction to Enzymes!
Web-Based Activity:Guided Notes on Enzymes
Go to “LewPort enzymes” (first link on google)
http://www.lew-port.com/10712041113402793/lib/10712041113402793/Animations/Enzyme_activity.html
Read aloud the blue text on each slide Use this info to fill in the Notes column on
the back of your worksheet Then use the animations on the Enzyme
Menu to fill in drawings or descriptions in the Evidence column
We will do the first one together… You will work quietly w/ your assigned
partner to complete the rest.Objectives for Class:
Describe what an enzyme does and how it works Explain why an enzyme’s shape is so important
Kenny
Emiliano
Yamile
Lennin
Martin
Amrita
Javier
Lorena
Lila
Imani
Nathy
Jesse
Hajar
BryanVictor
Martely
Daysia
Devin
Naidaliz
Joshua
Joelle
Cassandra
BeverlyErick
OliviaAlerte
Web-Based Activity:Guided Notes on Enzymes
Go to “LewPort enzymes” (first link on google)
http://www.lew-port.com/10712041113402793/lib/10712041113402793/Animations/Enzyme_activity.html
Read aloud the blue text on each slide Use this info to fill in the Notes column on
the back of your worksheet Then use the animations on the Enzyme
Menu to fill in drawings or descriptions in the Evidence column
We will do the first one together… You will work quietly w/ your assigned
partner to complete the rest.Objectives for Class:
Describe what an enzyme does and how it works Explain why an enzyme’s shape is so important
Homework: Now: Take out Intro to Enzyme worksheet Tonight: Starch Digestion Pre-Lab Questions (pages 2-
3 #1-6)Silent Do Now: (in NB w/ title & date)Describe or define each of the following terms.
What does each one mean to you? If you’re not sure, write what you think. What questions do you have about them? How do these terms relate to each other? Chemical reaction Enzyme Reactant Product
Bio 9A/9D: Wednesday, 11.3.10Title: ENZYMES
You should be writing silently for the entire Do Now time.
Today’s Objectives & Agenda
Objectives: Describe and illustrate chemical reactions,
reactants, products, and enzymes Explain how enzymes work to catalyze
reactions Use a knowledge of enzymes to predict what
will happen in tomorrow’s lab Agenda:
Notes: Vocabulary & Example Pre-Lab for tomorrow’s lab Start HW
Objectives for Class: Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab
Chemical Reaction:Process that changes or transforms one substance into another
Either to build something from smaller pieces – Synthesis
Or to break something down – DecompositionEx: glucose + O2 CO2 + H2O + ATP
energy + heat Enzyme:
A protein that speeds up chemical reactions in living things (the “catalysts” in our cells and bodies)
Chemical Reaction Vocabulary:
Chemical Reaction Vocabulary continued:
Reactants:the starting materials in a chemical reaction(also known as substrates)
Products:substances that are produced in the chemical reaction (the end result)
Objectives for Class: Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab
The enzyme lipase helps to break down lipids into their building block molecules (fatty acids and glycerol)
Enzyme – Reactant (aka Substrate) – Products – Lipids Fatty acids +
glycerolSubstrate/ Reactant Products
LipaseEnzyme helps
substrate turn into products
Example: Lipid Digestion
Objectives for Class: Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab
Lipase Lipi
dsFatty acids and glycerol
Which enzyme is best structured to help break down lipids? Why?
Example: Lipid Digestion
1 32Objectives for Class:
Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab
Example: Lipid Digestion
2
Which enzyme is best structured to help break down lipids? Why?
The SHAPE of an enzyme must match the SHAPE of its specific substrates!Objectives for Class:
Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab
Enzyme Lab Preview:Starch Digestion
Background Info… Procedure
Summary… Work w/ partner
to answer pre-lab questions #1-6
HW: Finish the pre-lab questions!
Starch & Water
Starch & Water & Enzyme(salivary amylase)
Objectives for Class: Describe and illustrate chemical reactions, reactants, products, and enzymes Explain how enzymes work to catalyze reactions Use a knowledge of enzymes to predict what will happen in tomorrow’s lab
Homework:Enzyme Lab Analysis Questions (typed or neatly
written on separate paper) Silent Do Now: (in notebook w/ title & date)
1. In 1-2 sentences, explain what this chemical equation means:
STARCH GLUCOSE2. Identify the reactant, product, and enzyme
in this reaction.3. Which two substances will we add to the
test tube at the beginning of our experiment?4. Which substance will be in the test tube at the
end of the experiment that wasn’t there at the beginning?
Bio 9A/9D: Thursday, 11.4.10Title: Enzyme Lab: Starch Digestion
salivary amylase
Today’s Objectives & Agenda Today’s Objectives:
Identify the reactant, product, and enzyme in a chemical reaction
Predict how an enzyme affects the speed of a chemical reaction
Use proper lab procedures to test how saliva enzymes affect the speed at which starch breaks down into glucose
Today’s Agenda: Review Do Now & Lab Procedures Do the lab!
1 2
Enzyme Lab: Starch Digestion STARCH GLUCOSE
Procedure
Prediction:
Results: How will we know?
Starch & Water
Starch & Water & Enzyme(salivary amylase)
Which tube will contain glucose after 10 minutes? Fill in the Prediction row on your lab table right now.
salivary amylase
*Stir the starch well before using it!
Benedict’s test
Enzyme Lab: Starch Digestion STARCH GLUCOSE
Safety: Goggles on! Be careful around hot plates! Stay at your own lab station, do not talk across
the room No eating/drinking
Clean Up: Pour test tubes into waste container Rinse test tubes in sink Put tray containing all materials on side counter Turn off hot plates if everyone at your table is
done If finished early: discuss Analysis Question #2
with your partner
salivary amylase
Kenny
Emiliano
Yamile
Lennin
Martin
Amrita
Javier
Lorena
Lila
Emiliano
Nathy
Jesse
Hajar
BryanVictor
Martely
Daysia
Devin
Naidaliz
Joshua
Joelle
Cassandra
BeverlyErick
OliviaAlerte
Kenny
Yamile
LenninMartin
Amrita
Javier
Lorena
Lila
EmilianoNathy
Jesse
Hajar
Bryan
Victor
Martely
Daysia
Devin
Naidaliz
Joshua
Joelle
Cassandra
Beverly
Erick Olivia
9A – New Seats You are sitting with your partner from the Enzyme Lab
Homework:Type up the Background Info and Purpose &
Hypothesis sections of your Denaturing Enzymes lab report (see rubric for details)
Silent Do Now:Complete questions 1 and 2 on the Denaturing Enzymes packet.
Today’s Objectives: Define and illustrate denaturing and explain how it
affects the function of enzymes Develop a specific experimental question and
hypothesis related to denaturing enzymes
Bio 9A: Monday, 11.8.10Title: Enzyme Lab Extension
Quick Review: Enzyme-Catalyzed Reactions
Objectives for Class: Define and illustrate denaturing and explain how it affects the function of enzymes Develop a specific experimental question and hypothesis related to denaturing enzymes
Enzyme = Protein that “Speeds Up” a Chemical Reaction
Enzymes are a “catalyst”
Products = Stuff Produced from a Chemical Reaction “Enzyme – Substrate
Complex”
Substrate = Reactants of a Chemical Reaction
“Active Site” – where the substrate bonds to the enzyme
This shows the reaction happening!!!
Denaturing EnzymesWhen an enzyme is denatured, it… Becomes misshapen and deformed Loses its original shape Becomes unable to speed up the
reaction it is designed for, because its shape no longer matches its substrate
Design Your Own Experiment
Work with your partner to complete pages 2 and 3 of the Denaturing Enzymes Lab Packet Purpose and Hypothesis Background Information
If you finish early, move on to the Procedure section (page 4)
HW: Write up the Background Info and Purpose & Hypothesis sections of the lab report See rubric for details Type if possible (and bring electronic copy
on Wed.)Hand in Analysis Questions at the end
of today’s class
Background Info Brainstorm Enzyme Denature Salivary amylase Heat or pH (acid/base) (depending on
your question) Glucose Substrates Products Chemical reaction Starch
Homework:Write (type if possible) the Procedure section of
your Denaturing Enzymes lab report (see rubric for details)
Silent Do Now: (in NB w/ title and date) Summarize what it means for an enzyme to be
denatured. Is this generally a good thing or a bad thing? Why?
Describe the experiment you are planning. How will you denature the enzyme? What do you think will happen?
Today’s Objectives: Write a clear, specific, and replicable procedure for carrying
out an experiment relating to denaturing enzymes Design a clear data table for recording the results of an
experiment
Bio 9A: Wednesday, 11.10.10Title: Denaturing Enzymes Lab (Day 2)
Revised Work Policy You must turn in the original assignment
and rubric, along with your revisions Posters: Redo OR just add missing info Lab Reports: Type revisions and reprint Staple them all together
Revisions due FRIDAY if you want them to show up on your 1st quarter report card
Otherwise, they still count towards raising your 1st quarter grade but won’t show up until the next report card
Denaturing Enzymes Lab:Procedure & Data Table
Work with your partner to complete pages 4 and 5 of the Denaturing Enzymes Lab Packet Plan your Procedure by answering the questions Get Dickson’s approval Write the Steps and Materials for your
procedure Write Materials List on clipboard on kidney
On page 6: Create your data table HW: Write up the Procedure section of the
lab report See rubric for details Type if possible (and bring electronic copy on
Friday) Friday: Do the experiment, then laptops for
typing!
Kenny
Imani
Yamile
Lennin
Martin
Amrita
JavierLorena
Lila
Emiliano
Nathy
Jesse
Hajar
BryanVictor
Martely
Daysia
Devin
Naidaliz
JoshuaJoelle
Cassandra
Beverly
Erick
Olivia Alerte
Homework:Finish typing the Lab Report (all sections) for the
Denaturing Enzymes Lab Silent Do Now:
Take out the Denaturing Enzymes Lab packet On page 6, create a data table for recording the
results of your experiment. Remember to include: Space to record results from all of your test tubes Space to record observations as well as +/- results
Bio 9A: Friday, 11.12.10Title: Denaturing Enzymes Lab (Day 3)
Today’s Objectives & Agenda Today’s Objectives:
Design a clear, specific, and replicable procedure and a clear data table for your experiment
Carry out your experiment Write a formal lab report
Agenda:1. Procedure Checklist (do this silently while Dickson
sets up groups)2. Get final procedure approved3. Gather materials4. Carry out experiment5. Discuss results w/ Ms. Dickson6. Type Lab Report (sit at front desks)
Kenny
YamileLennin
Martin Amrita
JavierLorena Lila
Emiliano
Nathy
Jesse
HajarBryan
Victor Martely
Daysia
Devin
Naidaliz Joshua
Joelle
Cassandra
Beverly
Erick Olivia
9A – New Seats for today
Alerte
Imani
Homework:Revise your lab report based on today’s peer
editing. Final draft due tomorrow, with rubric! Silent Do Now:
Take out your lab report On the top, write:
1. What is one thing you think you have done well in this rough draft?
2. What is one thing you think you could improve? What would you most like feedback/suggestions on?
Bio 9A: Monday, 11.15.10Title: Denaturing Enzymes Lab Wrap-Up
Today’s Objectives & Agenda Today’s Objectives:
Compare and contrast experimental results between different groups, and draw class-wide conclusions
Use the lab report rubric and peer editing sheet to give and receive helpful feedback on the Denaturing Enzymes lab report
Agenda:1. Groups report out on results2. Peer editing of lab reports
In your notebook set up a place for Denaturing Enzyme Lab Class Results How does HEAT affect enzymes?
How does extreme pH affect enzymes?
Class Results:How does HEAT affect enzymes?
Group No enzyme Room Temp (25oC)
Boiling Water (100oc)
Joshua, Javier, Erick
+ Slight + / Slight -
Yamile, Hajar, Olivia
- + +
Lila, Devin
- + Slight +/-
Daysia, Victor
- + Slight +
Cassandra &
Nathy
- + -
+ = positive result (glucose was present, reaction occurred)– = negative result (no glucose present, reaction didn’t occur)
In your notebook set up a place for Denaturing Enzyme Lab Class Results How does HEAT affect enzymes?
Boiling enzymes denatures some enzymes but not all
Maybe needed to boil them longer Heat doesn’t make enzymes completely
useless, just less effective How does extreme pH affect enzymes?
Lemon juice (acid) and bleach (base) denature enzymes
Hydrochloric acid is not consistent but using more works better
Maybe household items work better than pure acids?
Class Results:How does pH affect enzymes?+ = positive result (glucose was present, reaction occurred)– = negative result (no glucose present, reaction didn’t occur)
Group No enzyme
Low pH (ACID)
Neutral pH High pH (BASE)
Martely, Emiliano
Slight + Lemon juice:
Complete -
+
Jesse, Martin
Slight + + Bleach:Complete -
Joelle, Lorena
Slight + HCl:-
+
Lennin, Bryan
Slight + HCl:Slight +
+
Amrita,
Kenny
Slight + 2 mL: +4 mL:
slight +
+
Today’s Objectives & Agenda Today’s Objectives:
Compare and contrast experimental results between different groups, and draw class-wide conclusions
Use the lab report rubric and peer editing sheet to give and receive helpful feedback on the Denaturing Enzymes lab report
Agenda:1. Groups report out on results2. Peer editing of lab reports – this is silent
individual work time1. Switch lab reports with your assigned partner.2. Read the lab report and evaluate each section
(just circle boxes, don’t give points/grades)3. Fill out the feedback form on the back of the
rubric.4. Last 5 min. of class = time for quiet partner
discussion
Homework:Study for tomorrow’s quiz Silent Do Now: (in NB w/ title and date)
Take out your lab report and rubric and put it on the corner of your desk
Take out your assignment sheet and find the study guide on the back.
Answer questions 1 and 2 in your notebook (refer to notes from 10/20/10 if you can’t remember)
Bio 9A: Tuesday, 11.16.10Title: Quiz Review: Carbon Compounds & Enzymes
Quiz Review: Carbon Compounds & EnzymesToday’s Objectives & Agenda Today’s Objectives:
Review important information for tomorrow’s quiz Identify specific topics that require additional
review Agenda:Work individually or with a partner to quietly
complete the study guide If you want to work with a partner, raise your
hand and ask. Write answers to all study guide questions in
your notebook. If noise levels get too loud… 3rd warning = silent
individual work Raise your hand if you need to move across the room Work silently or talk quietly with your partner only
Homework:Read Section 38.2 and complete the reading
guide Do Now:
Clear your desks and get quiet for the quiz When Finished with the Quiz:
Get a textbook and start the HW Today’s Objectives:
Demonstrate what you know about carbon compounds and enzymes on the quiz
Bio 9A: Wednesday, 11.17.10Title: Quiz: Carbon Compounds & Enzymes