Opportunities for cooperation between ChinAquaNet and Ghent University

Ghent, August 31, 2007

UGENT AQUACULTURE R&D CENTRE

Research at the Lab. of Aquaculture& Artemia Reference Center

Research at the Lab. of Aquaculture& Artemia Reference Center

Peter BossierPatrick Sorgeloos

Peter BossierPatrick Sorgeloos

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Artemia research

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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A. persimilis

A. franciscana monica

A. urmiana

A. sp. (unidentified population)A. parthenogeneticaA. franciscanaA. salinaA. sinica

A. tibetiana

World distribution of Artemia species• bisexual species : A. salina, A. urmiana, A. sinica, A. tibetiana, A. franciscana,

A. persimilis• parthenogenetic populations

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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established in 1978 upon suggestion of the FAO

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Genetic fingerprinting patterns (RFLP)for Artemia species authentication

RFLP HpaII

100 bp DNA ladder

A. salina

A. persimilis

parthenogenetic Art.

parthenogenetic Art.

A.franciscana

A. franciscana

A. franciscana

A. franciscana

A. franciscana

A. sinica

A. sinica

Mégrine, Tunisia

Argentina*

Aibi Lake, Xinjiang, China

Vineta Swakopmund, Namibia

Vinh Chau, Vietnam

San Francisco Bay, California, USA

Vinh Chau, Vietnam

Macau, Brazil

Great Salt Lake, USA

Xiechi Lake, Yuncheng, Shanxi, China

Yimeng area, Inner Mongolia, China*

1268

1321

1236

1186

1301

1258

1456

1300

1287

1218

1188

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Developing new tools for Artemia population studies:

•In a lot of Artemia site, populations seem to contain more than one species or strain

•Commercial products can contain Artemia cysts from different origin

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Bohai bay, China

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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DGGE on Artemia from Bohai Bay:

studying mixed species/strain populations

parth A. sinica

In each lane, each band represent a different rDNA genotype

A. franciscana

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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DGGE on Artemia:comparison with individual analysis

franciscana

parth

ARCcode

A. parth%

A. sinica

%

A. franc%

97936891

1001418979300

?

11251211161712191187161612401213123312611350

00060803900

10097

3732300253700

000006180003

sinica

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Nutritional research

Artemia enrichment

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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10-day old seabass larva feeding on Artemia

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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pigments

prophylactics

essential nutrients

Bioencapsulation or Enrichment

therapeutics

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Lipid emulsion in gut of Artemia

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Nutritional research

Brachionus genetic diversity

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Follow up of culture H

00.10.20.30.40.50.60.70.80.9

1

H0 H5 H8 H10 H12 H14 H16auscay

DGGE for studying mixtures of cryptic Brachionus species

Austria Cayman

H0 H5 H8 H10 H12 H14 H16

RFLP on individuals

DGGE on mixtures

17 consecutive batches (3 days )of Brachionus culturing on industrial scale

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Larviculture Research

Mud crab : Scylla spp.

Macrobrachium rosenbergii

Penaeid shrimp

Turbot

Sea bass

cobia

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Host-microbial interactions

Quorum sensing

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Quorum sensing in Vibrio harveyi

QUORUM SENSING (QS) = Bacterial cell-to-cellcommunication with signal molecules

LuxSLuxQ

LuxP

LuxM

LuxN

HAI-1 AI-2CAI-1

CqsS

CqsALuxU

LuxO

LuxR

sRNA’s + Hfq

Target genes

σ54

N O

OH

H OH O

B-

OH

OH

OH OH

HAI-1= AHL

AI-2

furanosyl borate diester

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Control

Wildtype

HAI-1 synthase

HAI-1 receptor

AI-2 synthase

AI-2 receptor

HAI-1 & AI-2 synthaseLuxO

Surv

ival

(%)

0

20

40

60

80

100

Artemia challenge with QS MUTANTS of Vibrio BB120

**

*

*: significantly different from control (P < 0,01)

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Effect of AHL on Turbot larvaesurvival in % on DAH7

Factor AHL addition

- +

-

(Treatment 1)

92.1 ± 8.3bc

(Treatment 2)

10.4 ± 10.0a

EC addition

EC3

(Treatment 3)

62.1 ± 24.4b

(Treatment 4)

2.1 ± 3.5a

EC5

(Treatment 5)

96.7 ± 4.0c

(Treatment 6)

94.3 ± 7.1bc

Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre

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Effect of AHL onMacrobrachium larviculture

Survival rate on day 8

77.7c77.1c77.3c

61.5bc

48.5ab

37.3a

31323334353637383

AHL+EC5 EC5 AHL+LVS3 LVS3 AHL Control

Treatment

Surv

ival

rate

(%)