downloadspro68-01 manual urin micro sop

8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP

1/19

Your Lab Name

Manual Urinalysis Microscopic Exam

Author: Penny Stevens

Document Number: Pro 68-0.1

Effective (or Post) Dte: ! "une #008

Document $ri%in

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S/E omments: 2his *ocument is rovi*e* s n e3me ony. t must be revise* to ccurtey refectyour b4s secific rocesses n*or secific rotoco re5uirements. &sers re *irecte* to counterchecfcts 7hen consi*erin% their use in other ictions. f you hve ny 5uestions contct S/E.

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U. URINALYSIS

U.02. MANUAL URINALYSIS MICROSCOIC E!AM

U.2.". RINCILE#

1. outine &rinysis consists of both hysic n* chemic nyses to ssist hysicinsin the *i%nosis n* tretment of ren n* urinry trct *iseses n* in the *etectionof metboic or systemic *isese rocesses not *irecty rete* to the i*ney.

#. 2he microscoic e3mintion of the centrifu%e* urine se*iment incu*es the stu*y offorme* eements+ such s ;


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Urinalysis

U.2. Manual Urinalysis Microscopic Exam

&o ,

C. (2he secimen must be brou%ht to room temerture before testin%.)

?. -'e *ollo%in urine samples are no& sa&is*ac&ory *or &es&in#

?.1. Secimens receive* over t7o hours fter coection.

?.#. /isbee* smes.

?.=. mroery coecte* smes. or e3me+ urine smes 7ith reservtives+secimens coecte* in non-sterie continers+ or secimens coecte* incontiners 7ith so or *eter%ent resi*ues 7i not be ccete*.

?.>. BNS (Buntity Not Sufficient) - 2he recommen*e* minimum voume is 1# m4s.2he re5uire* minimum voume for microscoic e3mintion is 0.?0 m. n theevent tht ess thn C0.?0 m is receive*+ erform testin% on uncentrifu%e* urinen* comment s note* in section &.#.@.

?.?. n the event tht n unccetbe sme is receive*+ nother sme must bere5ueste*. Document ction ten in the S.

U.2.+. E/UIMEN- REA1EN-S

1. E5uiment:

1.1 ov System - ov si*es+ iettes tubes1.#. entrifu%e 7ith timer1.=. /icroscoe 7ith 103 n* >03 obFectives1.>. ens er or int free tissues1 ? h


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Urinalysis


run.(see above)

1.3. econstitute ne7 ov contros 7ith 40 mL Reaen& 1ra5e %a&erccor*in%to mnufcturer4s instructions.

1.=.1. After reconstitution+ serte contros into # m i5uots usin%*isosbe trnsfer iettes n* stic test tubes. ti%htyH

1.=.#. be ech stic test tube 7ith Normalor Abnormal 6o7a Con&rol8ot number+ e3irtion *te+ reconstitution *te+ n* initis of tech 7horeconstitutes contros.

1.=.=. Sme i5uots 7i be store* froGen t -#0. 2he sme i5uots re

stbe t this temerture for up &o *our (+) mon&'s.1.=.>. $ny one freeGeth7 cyce is ermitte*. Ai5uots must be t room

temerture before testin%. Do not use 7rmin% boc to th7.1.=.?. Sme i5uots re stbe for one hour t 1?-#0I or #> hours t #-8I.

1.4. Perform the ov contros in the sme mnner s tient smes. See section&.#.@ for the tient testin% roce*ure. ecor* the resuts on the &rinysisBuity ontro ;orsheets. See en*i3 = >.

1.5. A resuts must f into the e3ecte* rn%es+ 7hich re obtine* from themnufcturer4s insert for ech contro n* recor*e* on the to of the &rinysisBuity ontro ;orsheets.

1.6. f resuts re 7ithin e3ecte* rn%es+ rocee* 7ith tient testin%.

1.7. f B resuts re outsi*e of e3ecte* rn%es for contros:

1 @ 1 i h i i *iff fi * f i


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Urinalysis


1. emove the secimenscontros from the refri%ertor or freeGer+ s icbe+ n*

o7 them come to room temerture for t est =0 minutes before mi3in%.

#. Positivey i*entify the secimens usin% the tient nme n* *te of birth.

=. o% the sme on the testin% 7orsheet usin% the S %enerte* tient be+en*i3 6.

>. A** the S %enerte* be to the sme cu (not the sme i*). Any 5iscrepancymus& be in7es&ia&e5 be*ore processin &'e specimen.

?. /i3 tient urine sme by s7irin%. /i3 contro i5uots by invertin% sever timesto ensure homo%eneity of the contents.

6. f mcroscoic e3mintion urine chemistry testin% is or*ere* refer to &.01.1/nu &rinysis Distic 2estin% S$P for testin% n* reortin% roce*ures. 2hemicroscoic e3mintion is erforme* after *istic testin% uness the rovi*erre5uests other7ise.

@. f intest+ Acetest or ctotest is re5uire* s iste* beo7 or re5ueste* by the rovi*er+erform testin% on uncentrifu%e* urine rior to erformin% the microscoic e3m.efer to in*ivi*u confirmtory test S$Ps s iste* in the references.

8. 2he Clini&es& con*irma&ory &es& is re5uire* for all pe5ia&ric urine samples 7ithtients %e2 years ol5 oryouner+ re%r*ess of 7hether it 7s re5ueste* or not.See initest S$P &06.1.

!. 3ac;up &es&s an5


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Urinalysis


11.#. /icroscoic e3mintion re5ueste* by the tient4s hysicin.

11.=. &rine secimens receive* from Pe*itric inic.11.>. theteriGe* n* Surubic sirtion smes.11.?. &rine secimens receive* from Physic E3m inic.

1#. Pour 1#-m4s of secimen into sterie ov tube n* be the tube 7ith the Ssecimen be. f the tot secimen voume is 0.?0 m or ess+ *o not our thesme into ov tube. Perform testin% from the secimen cu s in*icte* in thefoo7in% stes.

1=. 2estin%

1=.1. Loumes %reter thn 0.?0 m

1=.1.1. Perform Distic+ initest+ Acetest+ or ctotest s re5uire* orre5ueste*.

1=.1.#. the ov sme n* centrifu%e t 1?00 rm for ? minutes.1=.1.=. emove the sme from the centrifu%e n* *iscr* the c.1=.1.>. f =K SSA testin% is re5uire* or re5ueste*+ erform testin% on the

suerntnt ccor*in% to the &.10.1. =K SSA S$P.1=.1.?. Decnt n* *iscr* the suerntnt 7hie retinin% the se*iment eet

in the conic ortion of the ov tube. 2here 7i be sm voumeof fui* retine* bove the se*iment. 2he tot voume retine* in theov system tube fter *ecntin% the suerntnt shou* bero3imtey 0.? m.

1=.1.6. &se ov *isosbe iette to resusen* the se*iment eet.1=.1.@. Procee* 7ith testin% in ste 1=.=.

1= # Loumes ess thn 0 ?0 m s7ir the sme in the secimen cu to mi3 7e


6/19


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Urinalysis


Jest None i%ht+ /o*erte or /ny. eort ny

bu**in% yest or hyhe seen usin%comments s note* beo7.

2richomons None e7+ /o*erte or /ny. /y ony bereorte* if motie.

#. f bu**in% yest or hyhe re resent+ ** the foo7in% cnne* comments:

#.1. O


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Urinalysis


?.#. ritic vues must be reorte* in ccor*nce 7ith the bortory critic vueoicy.

?.#.1. A critic microscoic se*iment must be verifie* by the bortorysuervisor before reortin% to the hysicinnurse n* before certifyin%resuts in the S.?.#.#. A resuts must be re* bc by the hysicinnurse n* thenotifiction re* bc *ocumente* in the S.

?.#.=. 2he thoo%ist must so be notifie* 7ithin # hours n* 7i*etermine if thoo%ist revie7 is re5uire*.

U.2.. ROCE:URAL NO-ES#

1. NORMAL C>ARAC-ERIS-ICS OB ->E URINE#2he yeo7 coor of the urineis *ue r%ey to the i%ment urochrome n* sm mounts of urobiin n* uroerythrin.Norm urine is essentiy cer+ n* the resence of rticute mtter in

uncentrifu%e* urine nee*s to be e3ine* microscoicy. Norm urine hs fint+romtic o*or of un*etermine* source.

#. Microscopic Se5imen

#.1.


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Urinalysis


Amorhous &rtes Amorhous

Phoshtes

ystine None

&ric Aci* 2rie Phoshtes 2yrosine

cium $3te Ammonium


10/19

Urinalysis


?.11.#.#. Ba&&y cas&s# Are semitrnsrent or %rnur cyin*ers

continin% r%e hi%hy refrctive vcuoes or *roets. Lisibeft *roets re tri%yceri*es or choestero esters. 2hese recommony seen 7hen there is hevy roteinuri n* re feture of the nehrotic syn*rome.

?.11.#.=. Crys&al Cas&s# rystine incusion in semitrnsrent or%rnur cyin*er. 2hese csts in*icte *isosition of crysts inthe tubue or coectin% *uct.

?.11.=. Cas& imen&s#

?.11.=.1. >emolobin (3loo5) cas&s#Aers yeo7 to re* sometimesthe coor is very e n* *ifficut to interret. 2hese csts ressocite* 7ith %omerur *isese.

?.11.=.#. Myolobin cas&s#2hese cst re re*-bro7n in coor n* occur

7ith myo%obinuri foo7in% cute musce *m%e.

?.11.=.=. 3ilirubin an5 o&'er 5ru cas&s#. Cellular Cas&s#

?.11.>.1. Ery&'rocy&e (Re5 3loo5 Cell) cas&s# Semitrnsrent or%rnur cyin*ers continin% *istinct erythrocyte Disor*ers


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Urinalysis


center of the 7e cn cuse ir bubbes to form resutin% in sme *istortion

*urin% e3mintion.

U.2."0. AEN:ICES#

1. S$P Li*tion orm n* S$P hn%e ontro#. S$P Arov=. &rinysis Norm Buity ontro ;orsheet>. &rinysis Abnorm Buity ontro ;orsheet

?. Buity ontro Pre 2estin% ;orsheet6. Ptient esut orm@. &rine ryst Proerties

U.2."". REBERENCES#

1. Strnsin%er+ Susn .+ &rinysis n* .

#. 'ber+ /ery '.+ &rinry Se*iment: A 2e3tboo Ats+ Americn Society of inicPthoo%ist .

=. /utisti3 10 7ith S, Pc%e nsert+ . ov 2ro: 'umn &rinysis ontros Pc%e nsert+ 'ycor


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Urinalysis


Aen*i3 1

S$P LADA2$N

S$P NA/E:U.2. Manual Urinalysis Microscopic Exam

er n* secific tite n* rincie: yes noomments:

A necessry suies+ e5uiment+ n* mteris re iste*: yes noomments:

S$P is sufficienty *etie* to be un*erstoo* but not overy come3: yes noomments:

S$P te3t *e5utey *escribes rocessroce*ure: yes noomments:

S$P ccomishes urose: yes no

omments:

i * b (N 2i ) )


13/19

Urinalysis


Aen*i3 #S$P APP$LA

S,NA2&E DA2E

PEPAE

BA $$DNA2$

A


14/19

Your Lab Name

Aen*i3 =Urinalysis Dipstick and Microscopic Normal Control Log

Normal Control Lot#: Expiration: Month/Year:

Dipst ick Lot# Expirat ion: Date in use: Supervisor Review:

Dipst ick Lot# Expirat ion: Date in use: Review Date:

Color/Clarity

Glucose Bilirubin Ketones Specific

Graity B lood p! "rotein Urobilin# Nitrate

Leu#$sterase

%BC/&pf 'BC/&pf Crystals Casts (ec&

)nitials

Date ccepta!le

Ran"e:

$

%

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$

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$

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$%

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$(

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$*

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Comments:

efer to S$Ps &.1.1 n* &.#.1 for &rine Distic n* /icroscoic B resutin% roce*ures

#?8=##@@=.*oc P%e 999 of 1! Effective: Dte


15/19

Your Lab Name

Aen*i3 >Urinalysis Dipstick and Microscopic *bnormal Control Log

!normal Control Lot#: Expiration: Month/Year:

Dipst ick Lot# Expirat ion: Date in use: Supervisor Review:

Dipst ick Lot# Expirat ion: Date in use: Review Date:

Color/Clarity

Glucose Bilirubin Ketones Specific

Graity B lood p! "rotein Urobilin# Nitrate

Leu#$sterase

%BC/&pf 'BC/&pf Crystals Casts (ec&

)nitials

Date ccepta!le

Ran"e:

$%

&

'

(

)

*

+

,

$

%

&

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(

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+

$,

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$%

$&

$'

$(

$)

$*

$+

%,

%

Comments:

efer to S$Ps &.1.1 n* &.#.1 for &rine Distic n* /icroscoic B resutin% roce*ures



16/19

Your Lab Name

Aen*i3 ?Buity ontro Pre 2estin% ;orsheet

Reaen& Curren& Lo&Resul&

Curren& Lo&Accep&able

Rane

Ne% Lo& Resul& Curren& Lo&Accep&able

Rane

Accep&able

e*


17/19

Urinalysis


Aen*i3 6 - Ptient /nu &rinysis esut eort

Anyte Ptient esuts eference n%e

'yine sts P:

0-1 1-= =-? ?-10 10-#? #?-?0?0

0-1 P

$ther sts:

None P 2ye:

2ye:

Eithei es 'P 0-# #-? ?-10 10-#? #?-?0 ?0 0-# 'P

euocytes (;


18/19

Urinalysis


omments:


19/19

Your Lab NameAen*i3 @ - &rine ryst Proerties


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