data praktikum urin refisi sheet 2

8/12/2019 Data Praktikum Urin Refisi Sheet 2

1/22

100 mcg/ml 200 mcg/ml

510 0.178 0.324

515 0.180 0.329

520 0.179 0.333

525 0.180 0.333530 0.179 0.331

535 0.181 0.334

540 0.178 0.331

545 0.176 0.327

550 0.174 0.323

555 0.173 0.318

560 0.169 0.311

Kadar (mcg/ml) Serapan

19.828 0.052

49.569 0.096

99.138 0.187 b 1.7162E-03

148.707 0.269 a 1.4978E-02

198.276 0.355 r 9.9990E-01

297.414 0.526

20.690 0.056 23.90298 115.52914

51.724 0.113 57.11647 110.42548

103.448 0.206 111.30691 107.59697

155.172 0.276 152.09541 98.01731

206.896 0.397 222.60125 107.59089

310.345 0.581 329.81674 106.27423

107.57233

b 1.8096E-03

a 1.5660E-02

r 9.9863E-01

Persen

Recovery (%)

Perolehan % Recovery Rerata

Persamaan Kurva Recovery

Tabel Nilai Serapan Na Salisilat pada Berbagai Kadar untuk

Penentuan Kurva Baku

Tabel Nilai Serapan Na Salisilat pada Berbagai

Panjang Gelombang untuk Penentuan Maks

Panjang

Gelombang (nm)

Serapan

Persamaan Kurva Baku


Penentuan Kurva Recovery

Perolehan

Kadar (ug/ml)SerapanKadar (ug/ml)


2/22

1 300 08:05 1.03 0.072 31.13387 9.340

2 300 09:03 2.00 0.102 47.71211 14.314

3 330 10:03 3.00 0.184 93.02597 30.699

4 380 10:58 3.92 0.099 46.05429 17.501

5 100 11:56 4.95 0.261 135.57678 13.5580.584 314.06916 25.126

0.119 285.53225 22.843

7 180 14:04 7.02 0.540 289.75441 52.156

8 190 14:58 7.92 0.185 93.57858 17.780

9 100 17:05 10.03 0.372 196.91627 19.692

10 90 18:59 11.93 0.412 219.02059 19.712

0.590 317.38481 25.391

0.136 332.50393 26.600

12 70 00:00 16.95 0.502 268.75531 18.813

13 60 03:02 19.98 0.513 274.83399 16.490

14 70 05:58 22.92 0.390 206.86321 14.48015 40 06:59 23.93 0.223 114.57768 4.583

16 60 08:01 24.97 0.139 68.15861 4.090

17 40 08:57 25.90 0.136 66.50079 2.660

18 20 10:03 27.00 0.099 46.05429 0.921

19 40 10:57 27.90 0.122 58.76427 2.351

20 70 12:04 29.02 0.069 29.47605 2.063

21 10 12:54 29.85 0.083 37.21256 0.372

22 170 14:06 31.05 0.012 -2.02260 -0.344

23 60 15:03 32.00 0.070 30.02866 1.802

312.818

Kadar

Terekskresi (ug)

Total Kadar Terekskresi (ug)

Untuk data ke 6 dan ke 11 perhitungannya menggunakan data yang kedua, yaitu

data setelah dilakukan 5x pengenceran. Sedangkan untuk data ke 22, nilai kadar

yang terekskresi tidak digunakan dalam perhitungan total kadar terekskresi karena

nilai minus kadar bermakna bahwa sampel yang keluar lebih encer daripada blanko

6 80 13:08 6.08

11 80 21:15 14.20

Tabel Data Sampel Urin

NoVolume

Urin (ml)

Jam

Tampung

Waktu

(jam)Serapan

Kadar

(ug/ml)


3/22

1 1.03 9.340 9.340 303.477 416.632 113.154

2 2.00 14.314 23.654 289.164 346.939 57.775

3 3.00 30.699 54.352 258.465 287.086 28.621

4 3.92 17.501 71.853 240.965 241.337 0.373

5 4.95 13.558 85.411 227.407 198.4466 6.08 22.843 108.253 204.564 160.116

7 7.02 52.156 160.409 152.409 134.177

8 7.92 17.780 178.189 134.629 113.152

9 10.03 19.692 197.881 114.937 75.785

10 11.93 19.712 217.592 95.225 52.884

11 14.20 26.600 244.193 68.625 34.428

12 16.95 18.813 263.006 49.812

13 19.98 16.490 279.496 33.322

14 22.92 14.480 293.976 18.841

15 23.93 4.583 298.559 14.258

16 24.97 4.090 302.649 10.16917 25.90 2.660 305.309 7.509

18 27.00 0.921 306.230 6.588

19 27.90 2.351 308.580 4.237

20 29.02 2.063 310.644 2.174

21 29.85 0.372 311.016 1.802

22 32.00 1.802 312.818 0.000

b -0.08224

a 2.70473

r -0.99988

0.18940

3.65891

b -0.30356

a 2.36786

r -1.00000

0.69911

0.99126

Xu~ - Xu

(mg)

Ekstrapolasi

Xu~ - Xu (mg)

Residual Xu~ -

Xu (mg)

Persamaan Regresi (Data ke 4, 20, dan 21)

Kel (/jam)

Pers. Regresi Residual (Data ke 1,2 dan 3)

Kab (/jam)

t1/2ab(jam)

Perhitungan Data Sampel Urin Menggunakan Metode Sigma Minus

No t (jam) Xu (mg) Xu~ (mg)

t1/2el (jam)


4/22

1 0.00

2 1.03 1.03 0.52 9.340 9.039

3 2.00 0.97 1.52 14.314 14.807

4 3.00 1.00 2.50 30.699 30.6995 3.92 0.92 3.46 17.501 19.092

6 4.95 1.03 4.43 13.558 13.120

7 6.08 1.13 5.52 22.843 20.155

8 7.02 0.93 6.55 52.156 55.881

9 7.92 0.90 7.47 17.780 19.755

10 10.03 2.12 8.98 19.692 9.303

11 11.93 1.90 10.98 19.712 10.375

12 14.20 2.27 13.07 26.600 11.735

13 16.95 2.75 15.58 18.813 6.841

14 19.98 3.03 18.47 16.490 5.436

15 22.92 2.93 21.45 14.480 4.93716 23.93 1.02 23.43 4.583 4.508

17 24.97 1.03 24.45 4.090 3.958

18 25.90 0.93 25.43 2.660 2.850

19 27.00 1.10 26.45 0.921 0.837

20 27.90 0.90 27.45 2.351 2.612

21 29.02 1.12 28.46 2.063 1.848

22 29.85 0.83 29.43 0.372 0.447

23 32.00 2.15 30.93 1.802 0.838

1 22.92 4.937 b -0.06800

2 23.93 4.508 a 2.26642

3 24.97 3.958 r -0.97414

4 25.90 2.850

5 27.90 2.612

6 29.02 1.848

0.15660

4.42536

Perhitungan Data Sampel Urin Menggunakan Metode Mid Point

Time

No t (jam) t (jam) tm (jam)

Kel (/jam)

t1/2 (jam)

Xu (mg)Xu/t

(mg/jam)

Xu/t(mg/jam)

No t (jam)

Pers. Garis Data Ke 15-18, dan 20-21


5/22


510 0.178 0.324

515 0.180 0.329520 0.179 0.333

525 0.180 0.333

530 0.179 0.331

535 0.181 0.334

540 0.178 0.331

545 0.176 0.327

550 0.174 0.323

555 0.173 0.318

560 0.169 0.311

Kadar (mcg/ml) Serapan

19.828 0.052

49.569 0.096

99.138 0.187 b 1.762

148.707 0.269 a 1.4978

198.276 0.355 r 0.9999

297.414 0.526

20.690 0.056 -0.81827 -3.95493

51.724 0.113 -0.78593 -1.51946

103.448 0.206 -0.73314 -0.70871

155.172 0.276 -0.69342 -0.44687206.896 0.397 -0.62474 -0.30196

310.345 0.581 -0.52032 -0.16766

-1.18326

b 1.8096

a 1.566


Persen

Recovery (%)


Kadar (ug/ml) SerapanPerolehan

Kadar (ug/ml)





Penentuan Kurva Recovery

Panjang

Gelombang (nm)

Serapan




6/22

r 9.9863

Kadar (mcg/ml) Serapan19.828 0.052

49.569 0.096

99.138 0.187 b 1.762

148.707 0.269 a 1.4978

198.276 0.355 r 0.9999

297.414 0.526

20.690 0.056 0.05601 0.27069

51.724 0.113 0.11301 0.21849

103.448 0.206 0.20602 0.19915

155.172 0.276 0.27603 0.17788

206.896 0.397 0.39704 0.19190

310.345 0.581 0.58106 0.187230.20756

b 1.8096

a 1.566

r 9.9863


0.00000

Perolehan

Kadar (ug/ml)

Persen

Recovery (%)





Panjang

Gelombang (nm)

Serapan




Tabel Nilai Serapan Na Salisilat pada Berbagai Kadar untukPenentuan Kurva Recovery

Kadar (ug/ml) Serapan

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

Absorban

0.25

0.3

0.35

0.4

rban


7/22

510 0.178 0.324

515 0.180 0.329

520 0.179 0.333

525 0.180 0.333

530 0.179 0.331

535 0.181 0.334

540 0.178 0.331545 0.176 0.327

550 0.174 0.323

555 0.173 0.318

560 0.169 0.311

0

0.05

0.1

0.15

.

Abso


8/22

1 300 08:05 1.03 0.072 -0.82560 -0.248

2 300 09:03 2.00 0.102 -0.80902 -0.243

3 330 10:03 3.00 0.184 -0.76370 -0.2524 380 10:58 3.92 0.099 -0.81068 -0.308

5 100 11:56 4.95 0.261 -0.72115 -0.072

0.584 -0.54266 -0.043

0.119 -3.99812 -0.320

7 180 14:04 7.02 0.540 -0.56698 -0.102

8 190 14:58 7.92 0.185 -0.76315 -0.145

9 100 17:05 10.03 0.372 -0.65981 -0.066

10 90 18:59 11.93 0.412 -0.63771 -0.057

0.590 -0.53935 -0.043

0.136 -3.95115 -0.31612 70 00:00 16.95 0.502 -0.58798 -0.041

13 60 03:02 19.98 0.513 -0.58190 -0.035

14 70 05:58 22.92 0.390 -0.64987 -0.045

15 40 06:59 23.93 0.223 -0.74215 -0.030

16 60 08:01 24.97 0.139 -0.78857 -0.047

17 40 08:57 25.90 0.136 -0.79023 -0.032

18 20 10:03 27.00 0.099 -0.81068 -0.016

19 40 10:57 27.90 0.122 -0.79797 -0.032

20 70 12:04 29.02 0.069 -0.82725 -0.058

21 10 12:54 29.85 0.083 -0.81952 -0.00822 170 14:06 31.05 0.012 -0.85875 -0.146

23 60 15:03 32.00 0.070 -0.82670 -0.050

-2.523

Untuk data ke 6 dan ke 11 perhitungannya menggunakan data yang kedua, yaitu

data setelah dilakukan 5x pengenceran. Sedangkan untuk data ke 22, nilai kadar

yang terekskresi tidak digunakan dalam perhitungan total kadar terekskresi karena

nilai minus kadar bermakna bahwa sampel yang keluar lebih encer daripada blanko

Total Kadar Terekskresi (ug)

11 80 21:15 14.20

6 80 13:08 6.08

SerapanKadar

(ug/ml)

Kadar

Terekskresi (ug)

Tabel Data Sampel Urin

NoVolume

Urin (ml)

Jam

Tampung

Waktu

(jam)


9/22

0.056 0.113 0.206 0.276 0.397 0.581

Kadar (ug/ml)

Kurva Serapan Salisilat pada Berbagai kadar

untuk

Kurva Baku dan Kurva Recovery

Kurva bak

Kurva reco

Kurva Serapan Salisilat vs Panjang Gelombang (nm)


10/22

0 510 515 520 525 530 535 540 545 550 555

Panjang Gelombang


11/22

1 1.03 -0.248 -0.248 -2.275 445.427

2 2.00 -0.243 -0.490 -2.033 381.082

3 3.00 -0.252 -0.742 -1.781 324.2834 3.92 -0.308 -1.050 -1.472 279.686

5 4.95 -0.072 -1.123 -1.400 236.722

6 6.08 -0.320 -1.442 -1.081

7 7.02 -0.102 -1.544 -0.978

8 7.92 -0.145 -1.689 -0.833

9 10.03 -0.066 -1.755 -0.767

10 11.93 -0.057 -1.813 -0.710

11 14.20 -0.316 -2.129 -0.394

12 16.95 -0.041 -2.170 -0.353

13 19.98 -0.035 -2.205 -0.31814 22.92 -0.045 -2.251 -0.272

15 23.93 -0.030 -2.280 -0.243

16 24.97 -0.047 -2.328 -0.195

17 25.90 -0.032 -2.359 -0.164

18 27.00 -0.016 -2.375 -0.148

19 27.90 -0.032 -2.407 -0.116

20 29.02 -0.058 -2.465 -0.058

21 29.85 -0.008 -2.473 -0.050

22 32.00 -0.050 -2.523 0.000

b -0.07009

a 2.72121

r -0.99901

0.16143

4.29294

b -0.07003a 2.72335

r -1.00000

0.16127

4.29718t1/2ab(jam)

Kel (/jam)

t1/2el (jam)

Pers. Regresi Residual (Data ke 1-5)

Kab (/jam)

Persamaan Regresi (Data ke 5,6,8,9,16,17,18)

Ekstrapolasi Xu~ -

Xu (mg)Xu~ (mg) Xu~ - Xu (mg)No t (jam) Xu (mg)

Perhitungan Data Sampel Urin Menggunakan Metode Sigma Minus


12/22

very


13/22

560

C1 (100mcg/ml)

C2 (200 mcg/ml)


14/22

447.702

383.115 0.00

326.063 1 1.03 1.03 0.52 -0.248281.158 2 2.00 0.97 1.52 -0.243

238.123 3 3.00 1.00 2.50 -0.252

4 3.92 0.92 3.46 -0.308

5 4.95 1.03 4.43 -0.072

6 6.08 1.13 5.52 -0.320

7 7.02 0.93 6.55 -0.102

8 7.92 0.90 7.47 -0.145

9 10.03 2.12 8.98 -0.066

10 11.93 1.90 10.98 -0.057

11 14.20 2.27 13.07 -0.31612 16.95 2.75 15.58 -0.041

13 19.98 3.03 18.47 -0.035

14 22.92 2.93 21.45 -0.045

15 23.93 1.02 23.43 -0.030

16 24.97 1.03 24.45 -0.047

17 25.90 0.93 25.43 -0.032

18 27.00 1.10 26.45 -0.016

19 27.90 0.90 27.45 -0.032

20 29.02 1.12 28.46 -0.058

21 29.85 0.83 29.43 -0.00822 32.00 2.15 30.93 -0.050

b -0.04578

a 1.57889

r -0.99576

0.10544

6.57261

Pers. Garis Data Ke 3,6,8,10,12,17,20

t1/2 (jam)

Kel (/jam)

Residual Xu~ -

Xu (mg)t (jam) tm (jam) Xu (mg)No t (jam)

Perhitungan Data Sampel Urin Menggunakan Metode Mid


15/22


16/22


17/22

-0.240-0.251

-0.252

-0.336

-0.070

-0.282

-0.109

-0.161

-0.031

-0.030

-0.139-0.015

-0.012

-0.016

-0.029

-0.046

-0.034

-0.015

-0.035

-0.052

-0.010-0.023

3 1.48710

6.08 1.304

7.92 1.2957

11.93 1.016

16.95 0.8351

25.9 0.4548

29.02 0.26670

Xu/t

(mg/jam)

Point Time

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

3 6.08 7.

LogXu/t

(mg/jam)


18/22


19/22


20/22


21/22

Xu~ Kel Kab t1/2 Kel t1/2

1 312.818 0.161 0.650 4.29 0.110 6.57

2 284.364 0.172 0.422 4.04 0.111 6.26

3 228.891 0.150 1.590 4.59 0.150 4.49

4 426.407 0.160 0.630 4.34 0.084 8.24

5 350.263 0.157 0.310 4.42 0.144 4.82

6 295.010 0.313 0.558 2.22 0.209 3.31

7 474.542 0.154 0.180 4.49 0.341 2.03

8 344.679 0.149 0.395 4.65 0.168 4.12

9 378.733 0.160 0.520 4.33 0.120 5.78

10 243.896 0.390 0.330 1.75 0.110 6.40

11 329.170 0.326 0.310 2.12 0.112 6.19

Rerata 333.525 0.208 0.536 3.749 0.151 5.292

MPTSigma MinusKel


22/22

The plasma salicylate half life (t1/2)

fallowing therapeutic doses is 2 to 4.5 hours, but in over

doses, it increases to 18 to 36 hours (Done, 1960).

The plasma half-life of salicylate is approximately 2 to 3 hours

data praktikum urin refisi sheet 2

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