timetable - tohoku university official english website · 19th japanese medaka and zebrafish...

19th Japanese Medaka and Zebrafish Meeting

Timetable September 20th (Friday)

12:00 - Registration

12:55 - 13:00 Opening remark

13:00 - 14:12 Oral session 1 (12 min) x 6

14:12 - 14:30 Coffee break



16:00 - 16:45 Keynote lecture

16:45 - 18:15 Poster discussion

18:15 - 20:15 Mixer

September 21st (Saturday)




11:37 - 13:00 Lunch

Lunch will NOT be provided by the meeting


14:00 - 14:10 Closing remark

14:15 - 15:00 Community meeting (in Japanese)


Program September 20th (Friday) 12:55 - 13:00 Opening remark 13:00 - 14:12 Oral session 1 Chairs, Kazuhide Asakawa (NIG) and Hiromi Hirata (NIG) Oral-1 13:00-13:12 From the olfactory bulb to higher brain centers: a comprehensive axon projection map revealed by genetic single-neuron labeling in zebrafish Nobuhiko Miyasaka1, Ignacio Arganda-Carreras2, Noriko Wakisaka1, Miwa Masuda1, Uygar Sümbül2, H. Sebastian Seung2, Yoshihiro Yoshihara1 1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, Japan, 2Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, USA Oral-2 13:12-13:24 Expression and dephosphorylation of Kv7/KCNQ channels for acquiring the unique membrane properties of zebrafish Mauthner cells Takashi Shimazaki, Takaki Watanabe, Yoich Oda Department of Biological Science, Graduate School of Science, Nagoya University Oral-3 13:24-13:36 Serotonin regulation by the habenula is essential for adaptive problem solving in zebrafish Ryunosuke Amo1, Masakazu Agetsuma1, Masae Kinoshita1, Felipe Fredes1, Toshiyuki Shiraki1, Tazu Aoki1, Ryo Aoki1, 2, Masako Yamazaki1, Shin-ichi Higashijima3, Masaru Matsuda4, Maximiliano L Suster5, Koichi Kawakami5, Toshio Ohshima6, Hidenori Aizawa1, and Hitoshi Okamoto1, 6 1Laboratory for Developmental Gene Regulation, Brain Science Institute, RIKEN, Japan, 2Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3Okazaki Institute for Integrative Bioscience, NIPS, Japan, 4Center for Bioscience Research and Education, Utsunomiya University, Japan, 5Division of Molecular and Developmental Biology, National Institute of Genetics, Japan, 6Department of Life Science and Medical Bioscience, Waseda University, Japan


Oral-4 13:36-13:48 Non-invasive in vivo 3D imaging of medaka using ultra high resolution spectral

domain optical coherence tomography

Fanny Moses Gladys1, Yiheng Lim1, Masaru Matsuda2 and Barry Cense1

1Center for Optical Research and Education (CORE), Utsunomiya University, Japan, 2Center for Bioscience Research and Education, Utsunomiya University, Japan Oral-5 13:48-14:00 Analysis of neural/molecular mechanisms of mate-guarding behavior in small

fish, medaka

Saori Yokoi1, Teruhiro Okuyama1, Yasuhiro Kamei2, Yoshihito Taniguchi3, Satoshi

Ansai4, Masato Kinoshita4, Takeo Kubo1, Hideaki Takeuchi1 1Department of Biological Sciences, Graduate School of Science, The University of

Tokyo, 2National Institute for Basic Biology, 3Department of Preventive Medicine and

Public Health, School of Medicine, Keio University, 4Division of Applied Biosciences,

Graduate School of Agriculture, Kyoto University

Oral-6 14:00-14:12 Analysis of mechanism underlying brain growth accompanied by neurogenesis

using medaka fish (Oryzias latipes)

Yasuko Isoe1, Teruhiro Okuyama1,2, Masahito Hoki1, Yuji Suehiro1,3, Genki Yamagishi1,

Kiyoshi Naruse2, Masato Kinoshita4, Yasuhiro Kamei2, Atushi Shimizu5, Takeo Kubo1,

Hideaki Takeuchi1 1Department of Biological Sciences, Graduate School of Science, The University of

Tokyo, Tokyo, Japan, 2National Institute for Basic Biology, Okazaki, Aichi, Japan, 3Department of Physiology, Tokyo Women's Medical University School of Medicine,

Tokyo, Japan, 4Division of Applied Biosciences, Graduate School of Agriculture, Kyoto

University, Kyoto, Japan, 5Department of Molecular Biology, Keio University School of

Medicine, Tokyo, Japan,



14:30 - 15:42 Oral session 2 Chairs, Hironori Wada (NIG) and Tohru Ishitani (Kyushu Univ) Oral-7 14:30-14:42 Genome modifications mediated by CRISPR/Cas9 system in zebrafish Satoshi Ota, Yu Hisano, Atsuo Kawahara

Laboratory for Cardiovascular Molecular Dynamics, RIKEN Quantitative Biology Center

(QBiC), Suita, Osaka, Japan

Oral-8 14:42-14:54 Functional analysis of sphingosine-1-phosphate (S1P) receptors knockout zebrafish Yu Hisano, Satoshi Ota, Michiko Muraki, Atsuo Kawahara

RIKEN, Quantitative Biology Center (QBiC), Japan

Oral-9 14:54-15:06 Moving microtubule-mediated dorsal determination Hiromu Hino1, Ryoko Seki2, Takashi Shimizu1,2, Francisco Pelegri3, and Masahiko

Hibi1,2

1Graduate School of Science and 2Bioscience and Biotechnology Center, Nagoya

University, 3University of Wisconsin-Madison

Oral-10 15:06-15:18 Molecular mechanisms underlying segmentation border formation in zebrafish somitogenesis Chimwar Wanglar1, Taijiro Yabe2 and Shinji Takada1, 2

National Institute for Basic Biology, National Institutes of Natural Sciences, Sokendai

University, Okazaki, Aichi, Japan

Oral-11 15:18-15:30 Elastin gene sub/neo-functionalization and formation of teleost-specific outflow tract, “bulbus arteriosus”, in fish development and evolution Yuuta Moriyama1, Jun K Takeuchi1,2, Kazuko Koshiba-Takeuchi1,2 1Cardiovascular Regeneration, IMCB, Univ. of Tokyo, Japan, 2Biological Science, Grad.

Sch. of Science, Univ. of Tokyo, Japan


Oral-12 15:30-15:42 Tooth renewal by the coupled action of osteoblasts and osteoclasts in medaka pharyngeal teeth Akiko Mantoku, Masahiro Chatani, Keiji Inohaya and Akira Kudo

Dept. Biol. Info., Tokyo Inst. Tech

15:42 - 16:00 Coffee break 16:00 - 16:45 Keynote lecture Chairs, Hiroyuki Takeda (Univ Tokyo) and Hayato Yokoi (Tohoku Univ) Mechanisms of sex determination in zebrafish

John H. Postlethwait, Angel Amores, Jennifer Anderson, Cristian Cañestro, Adriana

Rodriguez, Samantha Steiner, Catherine Wilson, Yi-lin Yan

University of Oregon, Eugene, Oregon, USA

16:45 - 18:15 Poster discussion

16:45 - 17:30 Odd numbers

17:30 - 18:15 Even numbers 18:15 - 20:15 Mixer


September 21st (Saturday) 9:10 - 10:10 Oral session 3 Chairs, Misato Fujita (Toyo Univ) and Daisuke Kobayashi (Kyoto Pref Univ Med) Oral-13 9:10-9:22 Identification of bone stem cells during growth and regeneration of endo- and exo-skeletons in zebrafish Kazunori Ando1, Genbu Abe2, Akira Kudo1, Koichi Kawakami2, and Atsushi Kawakami1

1Department of Biological Information, Tokyo Institute of Technology, Yokohama,

Japan, 2Department of Developmental Genetics, National Institute of Genetics,

Mishima, Japan

Oral-14 9:22-9:34 Molecular and cellular analysis of blood vessel regeneration in zebrafish caudal fin Yoshiko Kametani1,2, Shinji Takada1, Didier Stainier2,3 1Division of molecular and developmental biology, Okazaki Institute for Integrative

Bioscience, National Institute for Basic Biology, 2University of California, San Francisco,

3Max Planck Institute for Heart and Lung Research

Oral-15 9:34-9:46 Differential actions of Fgf signaling controls blastema formation and regeneration in zebrafish Natsumi Horita1, Genbu Abe2, Koichi Kawakami2, Akira Kudo1, and Atsushi Kawakami1

1Department of Biological Information, Tokyo Institute of Technology, 2Department of

Developmental Genetics, National Institute of Genetics

Oral-16 9:46-9:58 Methylmercury detoxification mechanism by the exosomal secretory pathway in zebrafish embryos Shintaro Imamura1, Takeshi Yabu2, Kenji Ishihara1, Yumiko Yamashita1, Michiaki

Yamashita1 1National Research Institute of Fisheries Science, Yokohama, Japan, 2Nihon University,

Fujisawa, Japan


Oral-17 9:58-10:10 Potassium and cesium excretion via ROMKa potassium channel expressed in the gill ionocytes of Mozambique tilapia Fumiya Furukawa1,2,3, Soichi Watanabe3, Toyoji Kaneko3 1Department of Marine Biology and Environmental Sciences, Faculty of Agriculture,

University of Miyazaki, 2Institute of Cellular and Organismic Biology, Academia Sinica,

Nankang, Taipei 11529, Taiwan, Republic of China (ROC), 3Department of Aquatic

Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo

10:10 - 10:25 Coffee break 10:25 - 11:37 Oral session 4 Chairs, Hideaki Takeuchi (Univ Tokyo) and Shoji Fukamachi (Japan Women’s Univ) Oral-18 10:25-10:37 A proximal conserved 5’-flanking region is necessary to drive endogenous skeletal/cardiac muscle-specific expression of myosin heavy chain gene, MYH14, in zebrafish Bhuiyan Sharmin Siddique1, Shigeharu Kinoshita1, Shuichi Asakawa1 and Shugo

Watabe1,2

1Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences,

Univ. of Tokyo, 2School of Marine Biosciences, Kitasato University, Japan

Oral-19 10:37-10:49 Regulatory mechanisms of myosin heavy chain genes (MYHs) expression during skeletal muscle development in fish: transient and transgenic analyses of MYHs promoters in zebrafish embryos Md. Asaduzzaman1, Shigeharu Kinoshita1

, A.K. Shakur Ahammad1, Shuichi Asakawa1

and Shugo Watabe1,2 1Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences,

The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan, 2School of Marine

Bioscience, Kitasato University, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan


Oral-20 10:49-11:01 Tetraspanin 3c is required for migration and boundary formation of pigment cells

in zebrafish skin pattern formation

Shinya Inoue1, Dae-Seok Eom2, Kondo Shigeru1, Parichy M David2, Masakatsu

Watanabe1 1Graduate School of Frontier Biosciences, Osaka University, 2Department of Biology,

University of Washington

Oral-21 11:01-11:13 Positional cloning of three leucophore color mutants of medaka indicates

leucophores of medaka genetically closely related to xanthophores

Tetsuaki Kimura1,2, Yusuke Nagao3, Hisashi Hashimoto3 and Kiyoshi Naruse1,2,4 1National Institute for Basic Biology, Interuniversity Bio-Backup Project Center, Okazaki

444-8787, Aichi, Japan, 2Department of Basic Biology, School of Life Science, The

Graduate University for Advanced Studies (SOKENDAI), 3Bioscience and

Biotechnology Center and Division of Biological Science, Graduate School of Science,

Nagoya University, Furocho, Chikusaku, Nagoya 464-8601, 4National Institute for Basic

Biology, Laboratory of Bioresources, Okazaki 444-8585, Aichi, Japan

Oral-22 11:13-11:25 Naturally occurring variation of CYP xenobiotic enzyme alleles in medaka sheds a

new light on sexual dimorphism variation among human populations

Takafumi Katsumura1,2, Shoji Oda2, Hiroshi Mitani2, Shoji Kawamura2 and Hiroki Oota1 1Laboratory of Anatomy and Anthropology, Kitasato University School of Medicine,

Kanagawa, Japan, 2Graduate School of Frontier Sciences, Department of Integrated

Biosciences, University of Tokyo, Chiba, Japan

Oral-23 11:25-11:37 A novel giant DNA transposon Albatross in medaka fish

Yusuke Inoue1, Takumi Aikawa1, Mariko Kondo2, Shin-ichi Morishita3, Kiyoshi Naruse4,

Atsuko Shimada1, Akihiko Koga5, Hiroyuki Takeda1

1Grad. Sch. of Sci., Univ. of Tokyo, 2MMBS., Univ. of Tokyo, 3Dept. of Comp. Biol., Univ.

of Tokyo, 4Lab. of Biores., NIBB, 5Primate Res. Inst., Kyoto Univ.


11:37 - 13:00 Lunch

Lunch will NOT be provided by the meeting 13:00 - 13:48 Oral session 5 Chairs, Yuko Nishiwaki (OIST) and Akinori Kawamura (Saitama Univ) Oral-24 13:00-13:12 Japanese medaka: A non-mammalian model for studying sex-dependent

telomere attrition and longevity in vertebrate

Doris WT Au1 1Department of Biology and Chemistry, State Key Laboratory in Marine Pollution, City

University of Hong Kong, Kowloon, Hong Kong

Oral-25 13:12-13:24 A Novel factor required for ciliary motility in Kupffer’s vesicle

Daisuke Kobayashi1, Anshin Asano2, Takashi Nakakura2, Tatsuya Tsukahara3, Hiroyuki

Takeda3, Haruo Hagiwara2, Takahiko Yokoyama1 1Department of Anatomy and Developmental Biology, Graduate School of Medical

Science, Kyoto Prefectural University of Medicine, Kyoto, Japan, 2Department of

Anatomy, Teikyo University School of Medicine, Tokyo, Japan, 3Department of

Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo,

Japan

Oral-26 13:24-13:36 Dephosphorylation of Dishevelled mediated by Hipk2 and PP1c sustains Wnt

signal transduction

Nobuyuki Shimizu, Tohru Ishitani

Division of Cell Regulation Systems, Medical Institute of Bioregulation, Kyushu

University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan


Oral-27 13:36-13:48 Mib1 controls cell migration through negative regulation of p120ctn function by

its ubiquitination

Takamasa Mizoguchi1,2, Kazuya Hirose3, Shoko Ikeda2, Saori Watanabe2, Motoyuki

Itoh1,2 1Graduate School of Pharmaceutical Science, Chiba University, 2Faculty of

Pharmaceutical Science, Chiba University, 3Division of Biological Science, Graduate

School of Science, Nagoya University

Oral-28 13:48-14:00 Development of the lateral line canal system in zebrafish

Hironori Wada, Koichi Kawakami

National Institute of Genetics, Shizuoka, Japan

14:00 - 14:10 Closing remark 14:15 - 15:00 Community meeting (in Japanese)


Poster Presentation Poster discussion time, 16:45 - 18:15

16:45 - 17:30 Odd numbers 17:30 - 18:15 Even numbers

Poster-1 Transient analysis of torafugu MYHM2528-1 promoter in zebrafish embryos: the regulatory mechanism of its expression in relation to muscle hyperplasia Ahammad A K Shakur1, Shigeharu Kinoshita1

, Md. Asaduzzaman1, Shuichi Asakawa1

and Shugo Watabe1,2

1Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences,

The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan, 2School of Marine

Biosciences, Kitasato University, Minami, Sagamihara, Kanagawa 252-0373, Japan

Poster-2 CRISPR/Cas system – an efficient and simple tool for targeted genome editing in medaka Satoshi Ansai and Masato Kinoshita

Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University,

Kyoto, Japan

Poster-3 Analysis of the Hippo-Yap signaling pathway controlling the retinal cell differentiation Yoichi Asaoka, Hiroshi Nishina

Department of Developmental and Regenerative Biology, Medical Research Institute,

Tokyo Medical and Dental University

Poster-4 Effect of water temperatures for willow minnow sex differentiation-related genes in the early developmental stages Hiroshi Ashida1, Masato Kinoshita2, and Toru Kobayashi1

1Laboratory for Aquatic Biology, Graduate School of Agriculture, Kinki University

Nakamachi, Nara 631-8505, Japan, 2Division of Applied Biosciences, Graduate School

of Agriculture, Kyoto University, Kyoto 606-8502, Japan


Poster-5 Functional complementation between two classes of green sensitive opsin genes in teleost fish Ryuichi Ashino, Satoshi Kasagi, Hiroko Okuda, Shoji Kawamura

University of Tokyo, Graduate school of Frontier Sciences, Department of Integrated

Biosciences

Poster-6 Cone subtype-specific microRNAs as putative regulators for photoreceptor subtypes: evidence from primary transcripts localization and target prediction in medaka Yutaka Daido1, Takehiro G. Kusakabe1,2 1Graduate School of Natural Science, Konan University, Kobe, Japan, 2Department of

Biology, Faculty of Science and Engineering, Konan University, Kobe, Japan

Poster-7 Genome editing in medaka: targeted inactivation of TLS polymerase genes using TALENs Yoshihiro Fujikawa1, Tomoko Ishikawa-Fujiwara1, Kumi Nakamura1, Tetsushi Sakuma2,

Takashi Yamamoto2, Takeshi Todo1 1Graduate School of Medicine, Osaka University, Suita, Japan, 2Graduate School of

Science, Hiroshima University, Higashihiroshima, Japan

Poster-8 Effects of body-color mutations on vitality: an attempt to establish easy-to-breed see-through medaka strains by outcrossing Ayaka Ohshima1*, Noriko Morimura1*, Chizuru Matsumoto1*, Ami Hiraga1*, Ritsuko

Komine1, Tetsuaki Kimura2,3, Kiyoshi Naruse2 & Shoji Fukamachi1 (*equal contribution) 1Laboratory of Evolutionary Genetics, Department of Chemical and Biological Sciences,

Japan Women's University, Mejirodai 2-8-1, Bunkyo-ku, Tokyo 112-8681, Japan, 2Laboratory of Bioresources, National Institute for Basic Biology, The Graduate

University for Advanced Studies (SOKENDAI), Okazaki-shi, Aichi 444-8585, Japan, 3National Institute for Basic Biology, Interuniversity Bio-Backup Project Center,

Okazaki-shi, Aichi 444-8787, Japan


Poster-9 Characterization of domain organization and neurotransmitter properties in the dorsal spinal cord of zebrafish Chie Satou, Yukiko Kimura, Shin-ichi Higashijima

National Institutes of Natural Sciences, Okazaki Institute for Integrative Bioscience,

National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan

Poster-10 The role of commissural inhibitory neurons in spinal cord for locomotion Chie Satou1, Yukiko Kimura1, Shinichi Higashijima1 1Okazaki Institute for Integrative Bioscience

Poster-11 A DEAH-box RNA helicase Dhx37 regulates metabolism of glycine receptor alpha subunit RNA Hiromi Hirata1,2,3, Kazutoyo Ogino1, Kenta Yamada1, Sophie Leacock4 and Robert J.

Harvey4

1Center for Frontier Research, National Institute of Genetics, Mishima, Japan, 2Department of Genetics, Graduate University for Advanced Studies, Mishima, Japan,

3PRESTO, Japan Science and Technology Agency, 4Department of Pharmacology,

UCL School of Pharmacy, London, United Kingdom

Poster-12 Study on a light signaling pathway for circadian entrainment in zebrafish Jun Hirayama1, Atsuo Kawahara2, Hiroshi Nishina1 1Department of Developmental and Regenerative Biology, Medical Research Institute,

Tokyo Medical and Dental University, Tokyo, Japan, 2Laboratory for Cardiovascular

Molecular Dynamics, Quantitative Biology Center, RIKEN, Osaka, Japan

Poster-13 Motion prediction, tracking and stabilization of free-swimming zebrafish Takuma Ihara1, Gao Yanglei1, Shintaro Matsui1, Xlanfeng Fei1, Wataru Shoji2, Koichi

Hashimoto1 1Graduate School of Information Sciences, Tohoku University, 2Frontier Research

Institute for Interdisciplinary Sciences, Tohoku University


Poster-14 Gsdf mutation causes a male-to-female sex reversal in the medaka, Oryzias latipes Takuto Imai1,2, Kentaro Saino2, Masaru Matsuda2 1United Graduate School of Agricultural Science, Tokyo University of Agriculture and

Technology, Fuchu, Japan, 2Center for Bioscience Research and Education,

Utsunomiya University, Utsunomiya, Japan Poster-15 Positional cloning of a medaka ataxia mutant ro reveals a role of Contactin1 in neural network formation for motor learning Chikako Inoue1, Kouichi Shimizu1,2, Hiroki Miyamoto3, Akiko Goshima2, Yasuhiro

Kamei5, Shigenobu Yonemura4, Yutaka Hirata3, Atsuo Kawahara6, and Masahiko Hibi1,2

1Bioscience and Biotechnology Center and 2Graduate School of Science, Nagoya

University, 3Chubu University College of Engineering, 4RIKEN CDB, 5NIBB, 6RIKEN

QBiC

Poster-16 Characterization of a zebrafish mutant showing cone photoreceptor degeneration Maria Iribarne, Yuko Nishiwaki, Ichiro Masai

Okinawa Institute of Science and Technology Graduate University (OIST), Okinawa,

Japan

Poster-17 ATF6α/β-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish Tokiro Ishikawa1,2, Tetsuya Okada1,2, Tomoko Ishikawa-Fujiwara3, Takeshi Todo3,

Yasuhiro Kamei4, Shuji Shigenobu5, Minoru Tanaka6, Taro L. Saito7, Jun Yoshimura7,

Shinichi Morishita7, Atsushi Toyoda8, Yoshiyuki Sakaki9, Yoshihito Taniguchi10, Shunichi

Takeda2,11 and Kazutoshi Mori1,2

1Dept Biophys, Grad Sch Sci, Kyoto Univ, 2CREST, JST 3Dept Radiat Biol Med Genet,

Grad Sch Med, Osaka Univ, 4Spectrography Bioimaging Fac, 5Funct Genomics Fac, 6Lab. Mol Genet Reprod, NIBB, 7Dept Comput Biol, Grad Sch Front Sci, Univ of Tokyo, 8Comp Genomics Lab, NIG, 9Toyohashi Tech, 10Dept Prev Med Public Health, Sch

Med, Keio Univ, 11Dept Radiat Genet, Grad Sch Med, Kyoto Univ


Poster-18 Genome editing in medaka: targeted inactivation of DNA photolyase family genes using CRISPR system Tomoko Ishikawa-Fujiwara, Yoshihiro Fujikawa, Kumi Nakamura, Takeshi Todo

Department of Radiation Biology and Medical Genetics, Graduate School of Medicine,

Osaka University

Poster-19 Application of IR-LEGO (Infrared Laser-Evoked Gene Operator) to the model animals and plants as Collaborative research projects in NIBB Atsuko Shimada1, Takuya Kaneko1, Teruhiro Okuyama1,2, Yasuko Isoe1, Eiji Kimura3,

Aiko Kawasumi4, Chikashi Nagayama4, Misako Saida-Taniguchi2, Atsushi Shimizu3,

Hitoshi Yokoyama4, Yoshihiro Morishita5, Toshinori Hayashi6, Hiroko Urawa7, Koji

Tamura4, Masato Kinoshita8, Kiyoshi Naruse2, 9, Hiroyuki Takeda1, Hideaki Takeuchi1,

Yasuhiro Kamei2,9 1Dept. Biol. Sci., Grad. Sch. Sci., Univ. Tokyo, 2Natl. Inst. Basic Biology (NIBB), 3Iwate

Med. Univ., 4Dept. Dev. Biol. Neurosci., Grad. Sch. of Life Sci., Tohoku Univ., 5Dept.

Dev. Morphogeometry, CDB, Riken, 6Dept. Biomed. Sci., Facl. Med., Tottori Univ. 7Gifu-Shotoku Univ. 8Grad. Sch. Agric., Kyoto Univ., 9Sch. Life Sci., Grad. Univ. Adv.

Studies (SOKENDAI)

Poster-20 A transgenic medaka line with visible markers for genotypic and phenotypic sex Akira Kanamori and Keiko Toyama

Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya

464-8602, Japan

Poster-21 A cis-regulatory module downstream of hes6 gene controlled by T-box and Ets transcription factors drives the expression in presomitic mesoderm and tailbud of zebrafish embryos Akinori Kawamura1, Miki Hoshikawa1, Kenji Nakajo1, Hiroki Obara1, Daisuke Yokota1,

Shin-ichi Higashijima2, Shinji Takada2, Kyo Yamasu1 1Graduate School of Science and Engineering, Saitama University, Saitama, 2Okazaki

Institute for Integrative Bioscience, National Institutes of Natural Sciences, Aichi, Japan


Poster-22 Positional determination of cranial vessels related with developing oligodendrocytes Yohei Kawana, Misato Fujita

Department of Life Sciences, Toyo University, Gunma, Japan

Poster-23 Gene induction, tracing, and ablation of the targeted single endothelial cells using IR-LEGO system Eiji Kimura1, Tomonori Deguchi2, Yasuhiro Kamei3, Wataru Shoji4, Shunsuke Yuba2,

Jiro Hitomi1 1Department of Anatomy, Iwate Medical University, Iwate, Japan, 2Tissue Engineering

Research Group, Health Research Institute, National Institute of Advanced Industrial

Science and Technology, Osaka, Japan, 3Spectrography and Bioimaging Facility,

National Institute for Basic Biology Core Research Facilities, National Institute for Basic

Biology, Aichi, Japan, 4Frontier Research Institute for Interdisciplinary Sciences, Tohoku

University, Miyagi, Japan

Poster-24 Hindbrain V2a neurons play critical roles in providing excitations to zebrafish spinal locomotor circuits during swimming Yukiko Kimura1, Chie Satou1, Shunji Fujioka2, Wataru Shoji3, Keiko Umeda4, Toru

Ishizuka4, Hiromu Yawo4, and Shin-ichi Higashijima1 1NINS, NIPS, Okazaki Inst Integr Biosci, 2Grad Sch Info, Kyoto Univ, 3CIR, Tohoku

Univ, 4Grad Sch Life Sci, Tohoku Univ

Poster-25 Transcriptional regulatory mechanism of pou2 that controls a broad spectrum of zebrafish development Kana Kobayashi, Alam Khan, Mst. Shahnaj Parvin, Kyo Yamasu

Graduate School of Science and Engineering, Saitama University, Saitama, Japan

Poster-26 Global analysis of gene expression in head blood vessels in zebrafish Taiga Koitabashi, Misato Fujita

Department of life sciences, Toyo University, Gunma, Japan


Poster-27 Single gene transgenesis changed schooling behavior in medaka Shoko Kubota1, Shoji Fukamachi2, Hiroshi Mitani1, Shoji Oda1 1Department of Integrated Biosciences, The University of Tokyo, Chiba, Japan, 2Department of Chemical and Biological Sciences, Japan Women’s University, Tokyo,

Japan

Poster-28 Analysis of the β5(ψβ) globin gene in medaka Kouichi Maruyama1, Bing Wang1, Yuji Ishikawa1, Shigeki Yasumasu2, Ichiro Iuchi2 1National Institute of Radiological Sciences, Chiba, Japan, 2Sophia University, Tokyo,

Japan

Poster-29 Functional analysis of zebrafish cerebellar neural circuitry in classical conditioning Koji Matsuda1, Miki Takeuchi2, Kazuhide Asakawa3, Masayuki Yoshida4, Masamichi

Ohkura5, Nobuhiko Miyasaka6, Yoshihiro Yoshihara6, Junichi Nakai5, Koichi Kawakami3,

Masahiko Hibi1,2, Takashi Shimizu1,2 1Graduate School of Science and 2Bioscience and Biotechnology Center, Nagoya

University, 3National Institute of Genetics, 4Graduate School of Biosphere Science,

Hiroshima University, 5Brain Science Institute, Saitama University, 6RIKEN Brain

Science Institute Poster-30 Microscope systems for tracking zebrafish and light stimulation Shintaro Matsui1, Wataru Shoji2, Koichi Hashimoto1 1Graduate School of Information Sciences, Tohoku University, 2Frontier Research

Institute for Interdisciplinary Sciences, Tohoku University

Poster-31 Common sensory inputs and differential outputs of segmentally homologous reticulospinal neurons in zebrafish hindbrain Aoba Mishiro1, Masashi Tanimoto1, Yoichi Oda1 1 Division of Biological Science, Graduate School of Science, Nagoya University


Poster-32 A dietary supplement Protandim protects zebrafish larvae against oxidative stress by activating the Nrf2-ARE pathway Kanako Mizumoto1, Yuna Yamauchi1, Miho Takeuchi1, Brooks M. Hybertson2,3, Makoto Kobayashi1 1University of Tsukuba, Tsukuba, Japan, 2University of Colorado at Denver, Aurora, USA, 3LifeVantage Corporation, South Jordan, USA Poster-33 Analysis of developmental process of molecular clocks at the suprachiasmatic nuclei and pineal gland using flounder Paralichthys olivaceus as a model organism Makoto Mogi1, Susumu Uji2, Hayato Yokoi1 and Tohru Suzuki1 1Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, 2National Research Institute of Aquaculture, Fisheries Research Agency Poster-34 Identification of genes involved in the formation process in transverse vessels of the medaka Naoki Murata, Misato Fujita Department of life sciences, Toyo University, Gunma, Japan Poster-35 Sox5 is involved in fate specification of pigment cells in medaka Yusuke Nagao1, Tomoko Adachi2, 3, Atsushi Shimizu4, Ryoko Seki2, Chikako Inoue2, Yasuhiro Kamei5, Ikuyo Hara6, Tetsuaki Kimura6, Yoshihito Taniguchi7, Kiyoshi Naruse6, Robert N. Kelsh3, Yuko Wakamatsu2, Masahiko Hibi2, Hisashi Hashimoto2

1Grad. Sch. of Sci. and 2Biosci. Biotech. Ctr., Nagoya Univ., 3Dept. of Biol. Biochem., Univ. of Bath, 4Dept. of Mol. Biol., Sch. of Med., Keio Univ., 5Spectrography and Bioimaging Facility, and 6Lab. of Biores., NIBB, 7Dept. of Preventive Medicine and Public Health, Sch. of Med., Keio Univ. Poster-36 Genome editing in medaka: targeted inactivation of Rev1 gene by TALEN Kumi Nakamura1, Yoshihiro Fujikawa1, Tomoko Ishikawa-Fujiwara1, Tetsushi Sakuma2, Takashi Yamamoto2, Takeshi Todo1 1Graduate School of Medicine, Osaka University, Suita, Japan, 2Graduate School of Science, Hiroshima University, Higashihiroshima, Japan


Poster-37 Comprehensive analysis of gbx2 target genes suggests that gbx2 functions as a transcriptional activator or a repressor depending on tissues and target genes Yukiko Nakayama, Zhe Wang, Kana Kobayashi, Kyo Yamasu Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan Poster-38 Behavioral analysis of feeding and locomotion in juvenile medaka with GnRH2 neuron ablations made by the use of a two-photon microscope Kei Nishikawa1, Shigenori Nonaka2, and Yoshitaka Oka1 1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 2Laboratory for Spatiotemporal Regulations, National Institute for Basic Biology Poster-39 Reconstructing 3D image based on tissue sections of internally fertilized egg in Oryzias latipes Toshiyuki Nishimaki1, Yoshitaka Kimori2, Takafumi Katsumura1, Shoji Oda3, Tsunehiko Hanihara1, Hiroki Oota1 1Deparments of Anatomy, Kitasato University School of Medicine, 2National Institutes of Natural Science new field Incubation Center Imaging Science Research, 3Deparment of Integrated Biosciences Graduate School of Frontier Sciences, University of Tokyo Poster-40 Upregulation of leukemia inhibitory factor (LIF) in retinal ganglion cells during optic nerve regeneration in adult zebrafish Kazuhiro Ogai1, Kayo Sugitani2, Yoshiki Koriyama1, Hiroshi Nakashima2, Satoru Kato1 1Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan, 2Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan Poster-41 Dynamic analysis of GlyR and gephyrin Kazutoyo Ogino1 and Hiromi Hirata1,2,3 1Center for Frontier Research, National Institute of Genetics, Mishima, Japan, 2Department of Genetics, Graduate University for Advanced Studies, Mishima, Japan, 3PRESTO, Japan Science and Technology Agency


Poster-42 Different effects of acetylcholinesterase inhibitors on the locomotor activity in zebrafish larvae Masahito Sawahata1, Kazuya Taguchi1, Yasuhiko Izumi1, Akinori Akaike1,2, Toshiaki Kume1 1Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 2Graduate School of Pharmaceutical Sciences, Nagoya University Poster-43 Molecular mechanisms involved in vascular patterning in the central nervous system Tsubasa Shimizu, Misato Fujita Department of life sciences, Toyo University, Gunma, Japan Poster-44 Reexamination of functional roles of Mauthner cell in acoustically evoked C-starts of zebrafish Atsuko Sugimoto, Sena Yokomichi, Masashi Tanimoto, Yoichi Oda Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan Poster-45 Morphological and electrophysiological properties of homeotically transformed Mauthner cells in zebrafish Saki Sugimura1, Takaki Watanabe1, Masashi Tanimoto1, Yoichi Oda1 1Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan Poster-46 Periodic contraction of actomyosin network during convergence movements in zebrafish neurulation Makoto Suzuki National Institute for Basic Biology, Okazaki, Japan Poster-47 A novel mutation involved in the development of neural crest-derived tissues and organs in zebrafish embryos Yuki Ito, Kazuki Takahashi, Mami Yoshimura, Masataka Nikaido, Akinori Kawamura, Kyo Yamasu Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan


Poster-48 A new data-mining method to search for behavioral properties that induce coordinated movement and their involvement in social learning in medaka fish (Oryzias latipes) Takashi Ochiai1, Yuji Suehiro1,2, Katsuhiro Nishinari3, Takeo Kubo1, Hideaki Takeuchi1 1Department of Biological Sciences, Graduate School of Science, The University of

Tokyo, Tokyo, Japan, 2Department of Physiology, Tokyo Women's Medical University

School of Medicine, Tokyo, Japan, 3Research Center for Advanced Science &

Technology, The University of Tokyo, Tokyo, Japan

Poster-49 Speed-accuracy tradeoffs and decision making by individuals and dyads of zebrafish in colour discrimination tasks Mu-Yun Wang1,2 and Lars Chittka1 1Psychology Division, School of Biological and Chemical Sciences, Queen Mary,

University of London, 2presentt address: Department of Biological Sciences, Graduate

School of Sciences, University of Tokyo

Poster-50 Basement membrane-dependent control of axogenesis of cerebellar granule cells

Miki Takeuchi1, Shigenobu Yonemura2, Kazuhide Asakawa3, Koichi Kawakami3, Shinji

Takada4, Takashi Shimizu1 and Masahiko Hibi1

1Bioscience and Biotechnology Center, Nagoya University, 2RIKEN Center for

Developmental Biology, 3National Institute of Genetics, 4 Okazaki Institute for Integrative

Bioscience

Poster-51 Transgenic medaka for detection of polycyclic aromatic hydrocarbons Yoshihito Taniguchi1, Yumi Harada1,2, Masato Kinoshita3, Shoji Fukamachi2, Kazuyuki

Omae1 1Deapartment of Preventive Medicine and Public Health, Keio University, School of

Medicine, 2Department of Chemical and Biological Sciences, Faculty of Science, Japan

Women’s University, 3Division of Applied Biosciences, Graduate School of Agriculture,

Kyoto University


Poster-52 Searching for genes affecting visually-evoked startle response properties with medaka inbred strains Satomi Tsuboko1, Tetsuaki Kimura2, Minori Shinya3, Yuji Suehiro1, Teruhiro Okuyama1,

Atsuko Shimada1, Hiroyuki Takeda1, Kiyoshi Naruse2, Takeo Kubo1, Hideaki Takeuchi1 1The University of Tokyo, Tokyo, Japan, 2National Institute of Basic Biology, Okazaki,

Japan, 3National Institute of Genetics, Mishima, Japan

Poster-53 Flounder eye-sidedness can be experimentally randomized by transient treatment with a Nodal antagonist SB-431542 at somite stage Youhei Washio, Hayato Yokoi, Tohru Suzuki

Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural

Science, Tohoku University, Sendai, Japan

Poster-54 Expression analysis of genes encoding transcription factors, Mbo-1, 2, 3 in Oryzias latipes, medaka

Reina Watanabe1, Saori Yokoi1, Yasuko Isoe1, Satoshi Anzai2, Masato Kinoshita2,

Atsushi Ugajin1, Teruhiro Okuyama1, Takeo Kubo1, Hideaki Takeuchi1

1Department of Biological Science, Graduate school of Science, University of Tokyo,

Japan, 2Division of Applied Biosciences, Graduate School of Agriculture, Kyoto

University, Kyoto, Japan

Poster-55 ARF6 is necessary for the formation of glycinergic synapse and thus for proper escape behavior in zebrafish Kenta Yamada, Hiromi Hirata

Center for Frontier Research, National Institute of Genetics, Mishima, Japan

Poster-56 Development of cerebellar granule cell axons in zebrafish Shingo Yamaguchi1,2, Miki Takeuchi1, Kazuhide Asakawa3, Koichi Kawakami3,

Takashi Shimizu1,2 , Masahiko Hibi1,2 1Bioscience and Biotechnology Center, Nagoya University, 2Graduate School of

Science, Nagoya University, 3National Institute of Genetics


Poster-57 Mechanisms of position-dependent regeneration in zebrafish fins

Tohru Yano1,2, Masataka Okabe2, Koji Tamura1 1Graduate School of Life Sciences, Tohoku University, Sendai, Japan, 2Department of

Anatomy, The Jikei University School of Medicine, Tokyo, Japan

Poster-58 Microglial activation is regulated by independent two processes in irradiation-injured brain of medaka (Oryzias latipes) Takako Yasuda, Shoji Oda, Yusuke Hibi, Satomi Satoh, Natsumaro Kutsuna, and

Hiroshi Mitani

Department of Integrated Biosciences, Graduate School of Frontier Sciences, the

University of Tokyo, Kashiwa, Chiba 277-8562, Japan

Poster-59 An intercalation model in the zebrafish fin regeneration Yuki Yokota, Akira Kudo, and Atsushi Kawakami

Department of Biological Information, Tokyo Institute of Technology, Yokohama,

Kanagawa, Japan

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