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Supplementary Information
Comparative Embryotoxicity of Phenanthrene and
Alkyl-phenanthrene to Marine Medaka (Oryzias
melastigma)
Jingli MU 1,2,3, Juying WANG 1,2, Fei JIN 1,2, Xinhong WANG 3 , Huasheng HONG 3
1. Key Laboratory for Ecological Environment in Coastal Areas,State Oceanic Administration, Dalian 116023,
China;
2. Division of Marine Chemistry, National Marine Environmental Monitoring Center, Dalian 116023, China;
3. State Key Laboratory of Marine Environmental Science, Environmental Science Research Center, Xiamen
University, Xiamen 361005, China.
Control Phenanthrene 200 µg/L
Phenanthrene 800 µg/L Phenanthrene 400 µg/L
Retene 200 µg/L Retene400 µg/L
Retene 400 µg/L Retene 800 µg/L
Fig. S1. Developmental toxicity and BSD signs scoring of phenanthrene and retenen on marine medaka embryos. Body hemorrhaging (BH) was scored according to yes or no (0–1); pericardial edema (PE) was scored according to the ratio of edema fluid volume to intracapsular volume (0–4); yolk sac edema(YE) was scored according to the ratio of edema fluid volume to capsule volume (0–3); stretched heart (SH) was scored according to the stretched degree of heart (0–2); circulation reduction (CR) was scored according to blood flow velocity (0–3).
Control
Phenanthrene 200 µg/L Phenanthrene 400 µg/L Phenanthrene 800 µg/L
Phenanthrene 800 µg/LPhenanthrene 400 µg/LPhenanthrene 200 µg/L
Retene 400 µg/LRetene 100 µg/L Retene200 µg/L
Retene 200 µg/LRetene 100 µg/LRetene 100 µg/L Retene 400 µg/L
Phenanthrene 400 µg/L Phenanthrene 800 µg/LPhenanthrene 200 µg/L
Ret 200 µg/LRet 100 µg/L Ret 400 µg/L
Fig. S2. Developmental toxicity of phenanthrene and retene on marine medaka larvae.